The sequencing and assembly of the maize Nested Association Mapping (NAM) founder lines, selected to represent a broad cross-section of maize diversity.
Kelly Dawe, Doreen Ware, Matthew Hufford, Kevin Fengler, Candice Hirsch, Kapeel Chougle, Arun Seetharam, Michael Syring, Marcella K. Tello-Ruiz, Sharin Wei, Margaret Woodhouse, Jonathan Gent, Shujun Ou, David Hufnagel, Jianing Liu, Nancy Manchanda, Sarah Pedersen, Samantha Snodgrass
Assembly methods: PacBio sequencing, CANU1.8 assembly, scaffolds validated by BioNano optical mapping, and ordered and oriented using linkage data Construction of pseudomolecules: Pseudomolecules were constructed from superscaffolds using genetic maps; gap sizes are 100N
The length of scaffold which takes the sum length (summing from longest to shortest scaffold) past 50% of the total assembly size.
A contig is a contiguous consensus sequence that is
derived from a collection of overlapping reads.
A scaffold is set of a ordered and orientated contigs
that are linked to one another by mate pairs of sequencing reads.